Table 2.

Experimental runs for a central composite design for microwave-assisted digestion with the corresponding responses (means) for each MRM transition (peak area)

	Factors		Responses (peak area, m/z)*
	Time (min)	Enzyme: protein ratio	634.6 → 991.8	390.9 → 258.25	929.5 → 1116.5
1	30.0	1:20	3252.00 ± 3.18	9245.00 ± 2.12	178.00 ± 1.41
2	3.0	1:100	2555.00 ± 2.47	10,877.00 ± 1.73	108.00 ± 1.00
3	3.0	1:20	3384.00 ± 4.24	11,218.00 ± 2.82	175.00 ± 1.50
4	30.0	1:100	1273.00 ± 1.00	9631.00 ± 2.62	110.00 ± 1.41
5	3.0	1:10	3815.00 ± 3.18	13,303.00 ± 2.38	221.00 ± 2.82
6	16.5	1:100	1391.00 ± 1.76	10,136.00 ± 1.70	145.00 ± 4.27
7	30.0	1:10	3265.00 ± 3.30	10,050.00 ± 2.06	185.00 ± 1.70
8	16.5	1:20	2985.00 ± 2.12	10,351.00 ± 2.75	171.00 ± 2.98
9	16.5	1:10	4098.00 ± 1.29	10,649.00 ± 0.50	174.00 ± 1.70
10	16.5	1:20	2690.00 ± 4.24	10,419.00 ± 0.95	166.00 ± 2.44

*mean ± standard deviation (n = 2)