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. 2021 Jul 1;11:13694. doi: 10.1038/s41598-021-93014-0

Figure 3.

Figure 3

EA treatment attenuated cocaine-induced increases of ΔFosB and GluR2 levels in the NAc. After 2 h of REIN testing, the mice were sacrificed and NAc tissue was collected from the brain, for quantification of protein levels in the NAc. Figure 2A shows the experimental timeline. (A) Following the study group interventions, Western blotting examined ΔFosB and GluR2 expression. The NC group (without any treatment) was used to confirm the basal levels of protein. The original Western blot images are available in Supplementary Figure S10, with photographs of each membrane taken after cropping. Data were calculated as the percentages of the NC group and are expressed as the means ± S.E.M. (n = 5 mice per group). (B) Quantification of ΔFosB expression from (A). *p < 0.05 versus NC group; #p < 0.05 versus the CO group; +p < 0.05 versus the CO + EA group (one-way ANOVA with the LSD post-hoc test). (C) Quantification of GluR2 expression from Fig. 3A. **p < 0.01 versus NC group; #p < 0.05 versus the CO group; +++p < 0.001 versus the CO + EA group (one-way ANOVA with the LSD post-hoc test). Abbreviations: NC = Negative control; CO = No intervention; CO + EA = EA treatment; CO + EA + GBP = EA treatment followed by i.p. GBP (1 mg/kg) injection.