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. 2021 Jul 1;11:13694. doi: 10.1038/s41598-021-93014-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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EA or GBP alone did not affect ΔFosB and GluR2 expression in cocaine-naïve mice. (A) Timeline for the experimental procedures. (B, C) Cocaine-naïve mice were treated with EA or GBP (1 mg/kg i.p.). Levels of ΔFosB and GluR2 expression were examined by Western blotting (n = 5 per group). The original Western blot images are available in Supplementary Figure S11 and S12, respectively. The bar graphs show the quantification of protein from (B, C). The same control group was used for both Western blotting assays. Abbreviations: Control = Cocaine-naïve mice; EA = cocaine-naïve mice with EA treatment; GBP = Cocaine-naïve mice with i.p. GBP (1 mg/kg) injection.