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. 2021 Jul 1;11:13673. doi: 10.1038/s41598-021-93117-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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AGR scanning of the second half of AQP6. (A) Approaching the second half of AQP6 in a similar manner to Fig. 2, peptide residues AQP6^158–167 when C-terminally attached to AGR results in a construct that reaches the PM (white arrows). (B) However, when residues AQP6^187–213 are C-terminally attached to AGR, the construct does not reach the PM. (C) Although, AQP6^232–276 residues (compromising AQP6 C-terminal) attached to AGR does localize to the PM. (D) Interestingly, when a scrambled version of peptide residues AQP6^187–213 are substituted for the native AQP^187–213 and C-terminally attached to AGR, this construct is able to reach the PM. Cells were observed microscopically using an upright Olympus BX51WI equipped with 100 × 1 NA objective and images were captured using a Grasshopper3 CMOS camera (FLIR, Richmond, BC, Canada) controlled by Leica LAS X version 3.5 software (available at: https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/). All figures were created using Adobe Illustrator CS6 (Available at: https://adobe.com/products/illustrator), under an Adobe Inc., Creative Cloud Desktop 2019 shared device license to Case Western Reserve University (CWRU) that operates until 3/31/2022. Scale bars: 8 µm.