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. 2021 Jul 1;11:13674. doi: 10.1038/s41598-021-93256-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The number of teeth in a graft at 19 days post -transplantation. We performed renal capsule transplantations of wild-type mouse mandibles together with cationized gelatin sheets containing (1) a negative-control siRNA, (2) Usag-1 siRNA or Runx2 siRNA, or (3) Usag-1 siRNA + Runx2 siRNA. The number of tooth structures was determined by histological evaluation of serial H&E sections and 3D micro-CT analysis. The number of tooth structures was confirmed by 3D reconstruction of serial H&E sections in representative samples. P = 0.020 for Usag-1 #304 vs. Runx2 Stealth siRNA; P = 0.656 for Usag-1 #903 vs. Runx2 Stealth siRNA. 3D images were reconstructed and analyzed using computer imaging software (VGSTUDIO MAX 3.2; Volume Graphics GmbH., Heidelberg, Germany). https://www.volumegraphics.com/en/products/vgstudio-max.htmlvolumegraphics.com. Additionally, 3D-VR image of H&E-stained sections were reconstructed and analyzed using computer imaging software (AVIZO 2019.2; Thermo Fisher Scientific, Waltham, MA, USA). https://www.thermofisher.com/jp/en/home/industrial/electron-microscopy/electron-microscopy-instruments-workflow-solutions/3d-visualization-analysis-software/avizo-materials-science.html.