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. 2021 Jul 1;11:13674. doi: 10.1038/s41598-021-93256-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical evaluation of serial sections and sqRT-PCR analysis from renal capsules transplanted with mandibles from E10 Runx2-KO mice and treated with Stealth siRNAs. (A-a, b) H&E staining showed no tooth structures; however, odontogenic epithelial-like cells with elongated, rectangular morphology were observed. Magnification: 200× and 400× . (A-c) Immunostaining for amelogenin was positive in odontogenic epithelial-like cells. Magnification: 400× . (A-d) Control. (B) sqRT-PCR analysis of the enamel-specific proteins, amelogenin and ameloblastin. sqRT-PCR at 19 days post-transplantation, using wild-type and Runx2-KO mouse mandibles with Usag-1 Stealth siRNA #304 or PBS.