Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 1;12:4070. doi: 10.1038/s41467-021-24366-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Deconvoluted intact mass spectra of secreted, purified MUC1 reporter produced in HEK293^{KO C1GALT1}, HEK293^{KO COSMC,} ^{KI ST6GALNAC1}, HEK293^KO ^{GCNT1, ST3GAL1/2}, and HEK293^{KO GCNT1} cells. Reporters were treated with neuraminidase to remove sialic acids and reduce complexity, and digested by Lys-C followed by HPLC C4 isolation yielding the 157 amino acid MUC1 TR O-glycodomain fragment. All MUC1 O-glycoforms (Tn and T) showed a rather homogeneous mass comprising 32–35 HexNAc/HexHexNAc residues, and with 33 or 34 HexNAc/HexHexNAc being the most abundant peak. For all intact mass spectra experimentally determined and theoretically calculated masses were composed in Supplementary Table 4. b Site-specific O-glycopeptide LC–MS/MS analysis of MUC1 reporters after AspN digestion.