Figure 5.

Temporal increase of neutralization breadth against VOCs in association with durable IgG antibodies that are resistant to RBD mutations

(A) Timeline of sample collection for longitudinal analysis: moderate (n = 30 from 15 individuals) and severe (n = 30 from 15 individuals). Timing and number of samplings are indicated as line-connected circles: first (T1, light blue) and second (T2, yellow).

(B) Mutation-susceptible and mutation-resistant IgG titers from moderate (blue; n = 15) and severe (red; n = 15) groups were quantitated using ELISA. Data from the same individual are connected with lines. Statistical significance and fold reduction are indicated above columns; ^∗∗∗∗p < 0.0001 (Wilcoxon test).

(C) Decay slopes of mutation-susceptible and mutation-resistant IgG titers from T1 to T2 period are plotted. Data from the same sample are connected with lines. Statistical significance is indicated above columns; ^∗∗∗∗p < 0.0001 (Wilcoxon test).

(D) Cross-neutralizing titers to the indicated virus strains from moderate and severe groups are plotted (left, T1; right, T2). Data from the same sample are connected with lines (circles, WK-521; triangles, 501Y.V3; squares, 501Y.V2). Statistical significance and fold reduction are indicated above column as follows: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001 (Friedman test).

(E) Cross-neutralization breadth index in each plasma was expressed by dividing the VOC NT titers by the Wuhan NT titers. Data from the same individual are connected with lines (triangles, 501Y.V3; squares, 501Y.V2). Statistical significance and fold reduction are indicated above column as follows: ^∗p < 0.05 and ^∗∗p < 0.01 (Wilcoxon test).