REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
REGN10933	Hansen et al., 2020	N/A
C002	Robbiani et al., 2020	N/A
REGN10987	Hansen et al., 2020	N/A
COVA1-16	Brouwer et al., 2020	MT599835.1, MT599919.1
COVA1-18	Brouwer et al., 2020	MT599837.1, MT599921.1
CR3022	Meulen et al., 2006	DQ168569.1, DQ168570.1
HPR-conjugated goat anti-human IgG	Southern Biotech	Cat#2040-05; RRID: AB_2795644
Bacterial and virus strains
hCoV-19/Japan/TY-WK-521/2020	National Institute of Infectious Diseases	EPI_ISL_408667
hCoV-19/Japan/QHN002/2020	National Institute of Infectious Diseases	EPI_ISL_804008
hCoV-19/Japan/TY8-612/2021	National Institute of Infectious Diseases	EPI_ISL_1123289
hCoV-19/Japan/TY7-503/2021	National Institute of Infectious Diseases	EPI_ISL_877769
VSV pseudovirus bearing SARS-CoV-2 spike protein	Tani et al., 2021	N/A
Biological samples
SARS-CoV-2-infected convalescent patient blood sample	Tokyo-Shinagawa Hospital, Yokohama Municipal Citizen’s Hospital, Kawakita General Hospital, and Tokyo Center Clinic	N/A
Pre-pandemic healthy individual blood sample	Japanese Red Cross	N/A
Chemicals, peptides, and recombinant proteins
RBD (amino acids: 331–529)	In-house	MN994467
K417N mutant RBD	In-house	N/A
E484K mutant RBD	In-house	N/A
N501Y mutant RBD	In-house	N/A
K417N, E484K, N501Y triple mutant RBD	In-house	N/A
HiTrap Protein G HP Columns	Cytiva	17-0404-01
Bovine serum albumin	Sigma-Aldrich	A2153
Tween-20	Fujifilm	167-11515
Can Get Signal #2	TOYOBO	NKB-301
OPD substrate	Sigma-Aldrich	P8287
D-MEM (Low Glucose) with L-Glutamine and Phenol Red	Fujifilm Wako Pure Chemicals	041-29775
D-MEM (High Glucose) with L-Glutamine and Phenol Red	Fujifilm Wako Pure Chemicals	044-29765
Fetal bovine serum	Biowest	S1780-500
Geneticin	Thermo Fisher Scientific	10131-027
Penicillin/streptomycin	Thermo Fisher Scientific	15140-122
Vacutainer CPT tube	BD Biosciences	362761
Formalin	Fujifilm Wako Pure Chemicals	062-01661
Crystal violet solution	Sigma-Aldrich	V5265
Methylene blue	Fujifilm Wako Pure Chemicals	137-06982
Ni-NTA agarose	QIAGEN	30230
Critical commercial assays
Bright-Glo luciferase assay system	Promega	E2620
Expi293 expression system	Thermo Fisher Scientific	A29133
Elecsys Anti-SARS-CoV-2	Roche Diagnostics	518316181
Experimental models: Cell lines
VeroE6/TMPRSS2 cells	JCRB Cell Bank	JCRB1819
Software and algorithms
Graphpad Prism 9	Graphpad	N/A
Other
iMark microplate reader	Bio-Rad	168-1130J1
Epoch2	Biotek	EPOCH2NS
GroMax Navigator Microplate Luminometer	Promega	GM2000
cobas e 411 plus	Roche Diagnostics	N/A