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Gene position and isothermal reaction results. A) Nucleotide schematic for N gene of SARS-CoV-2 with primer pair indicating relative positions of CDC N1-3. Selected primer pair for RPA-LF (N1 Fw and N3 Rv) are highlighted in red boxes. Green square depicts the position of RPA-LF probe. B) RPA-basic assessment of primer combinations at 40 °C.
