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. 2021 Jul 2;296:114227. doi: 10.1016/j.jviromet.2021.114227

Fig. 4.

Fig. 4

Sensitivity of RPA-LF according to the incubation time at 42 °C. The assay has a limit of detection equivalent to 0.25 copies/μL of SARS-coV-2 since it was able to consistently amplify this cDNA concentration (5/6 total repetitions). Concentrations of 0.125 copies/μL yielded positive reactions in 2/3 repetitions.