Table 1.

qPCR and RPA-LF primers and Probes.

	5′-3′	Purpose
2019-nCoV_N1-F	GAC CCC AAA ATC AGC GAA AT	RT-qPCR
2019-nCoV_N1-R	TCT GGT TAC TGC CAG TTG AAT CTG	RT-qPCR
2019-nCoV_N1-P	FAM-ACC CCG CAT TAC GTT TGG TGG ACC-BHQ1	RT-qPCR
2019-nCoV_N2-F	TTA CAA ACA TTG GCC GCA AA	RT-qPCR
2019-nCoV_N2-R	GCG CGA CAT TCC GAA GAA	RT-qPCR
2019-nCoV_N2-P	FAM-ACA ATT TGC CCC CAG CGC TTC AG-BHQ1	RT-qPCR
2019-nCoV_N3-F	GGG AGC CTT GAA TAC ACC AAA A	RT-qPCR
2019-nCoV_N3-R	TGT AGC ACG ATT GCA GCA TTG	RT-qPCR
2019-nCoV_N3-P	FAM-AYC ACA TTG GCA CCC GCA ATC CTG-BHQ1	RT-qPCR
RP-F	AGA TTT GGA CCT GCG AGC G	RT-qPCR
RP-R	GAG CGG CTG TCT CCA CAA GT	RT-qPCR
RP-P	FAM – TTC TGA CCT GAA GGC TCT GCG CG – BHQ	RT-qPCR
RV2019-nCoV_N3 Biot	/5BiosG/TGTAGCACGATTGCAGCATTG	RPA-LF
FW2019-nCoV_N1	GAC CCC AAA ATC AGC GAA AT	RPA-LF
Probe2019-nCoV_N1ext	/56-FAM/ACCCCGCATTACGTTTGGTGGACCCTCAGAT/idSp/CAACTGGCAGTAACCAGA/3SpC3/	RPA-LF