REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block), Clone 2.4G2	BD Biosciences	Cat#553142; RRID: AB_394657
BD Optibuild BUV395 Rat Anti-Mouse CD44, Clone IM7	BD Biosciences	Cat#740215; RRID: AB_2739963
BD PharMingen PE Rat Anti-Mouse CD3 Molecular Complex, Clone 17A2	BD Biosciences	Cat#555275; RRID: AB_395699
BD Horizon BUV737 Rat Anti-Mouse CD19, Clone 1D3	BD Biosciences	Cat#612781; RRID: AB_2870110
BD Horizon BV711 Rat Anti-Mouse CD45R/B220, Clone RA3-6B2	BD Biosciences	Cat#563892; RRID: AB_2738470
BD Horizon BV605 Rat Anti-Mouse CD138, Clone 281-2	BD Biosciences	Cat#563147; RRID: AB_2721029
BD Optibuild BUV563 Hamster Anti-Mouse CD95, Clone Jo2	BD Biosciences	Cat#741292; RRID: AB_2870823
BD Optibuild BUV805 Rat Anti-Mouse CD4, Clone RM4-5	BD Biosciences	Cat#741912; RRID: AB_2871226
BD Horizon APC-R700 Hamster Anti-Mouse CD279 (PD-1), Clone J43	BD Biosciences	Cat#565815; RRID: AB_2739366
BD PharMingen APC-H7 Rat Anti-Mouse IgD, Clone 11-26c.2a	BD Biosciences	Cat#565348; RRID: AB_2739201
BD Horizon PE-CF594 Rat Anti-Mouse CD185 (CXCR5), Clone 2G8	BD Biosciences	Cat#562856; RRID: AB_2737842
BD Optibuild BUV737 Rat Anti-Mouse CD3 molecular complex, Clone 17A2	BD Biosciences	Cat#741788; RRID: AB_2871136
BD Horizon BV480 Rat Anti-Mouse CD4, Clone RM4-5	BD Biosciences	Cat#565634; RRID: AB_2739312
BD PharMingen PE Rat Anti-Mouse I-A/I-E, Clone M5/114.15.2	BD Biosciences	Cat#557000; RRID: AB_396546
BD Horizon BV421 Hamster Anti-Mouse CD279 (PD-1), Clone J43	BD Biosciences	Cat#562584; RRID: AB_2737668
BD Horizon BV605 Hamster Anti-Mouse CD69, Clone H1.2F3	BD Biosciences	Cat#563290; RRID: AB_2738120
BD Horizon BUV805 Rat Anti-Mouse CD8α, Clone 53-6.7	BD Biosciences	Cat#612898; RRID: AB_2870186
BD Horizon BV605 Rat Anti-Mouse CD62L, Clone MEL-14	BD Biosciences	Cat#563252; RRID: AB_2738098
BD Horizon BV650 Rat Anti-Mouse IFN-γ, Clone XMG1.2	BD Biosciences	Cat#563854; RRID: AB_2738451
BD Horizon BV711 Rat Anti-Mouse TNFα, Clone MP6-XT22	BD Biosciences	Cat#563944; RRID: AB_2738499
BD Horizon BV421 Rat Anti-Mouse IL-2, Clone JES6-5H4	BD Biosciences	Cat#562969; RRID: AB_2737923
PE/Cyanine7 anti-mouse/human GL7 Antigen (T and B Activation Marker) Antibody	Biolegend	Cat#144620; RRID: AB_2800677
Brilliant Violet 510 anti-mouse IgM Antibody, Clone RMM-1	Biolegend	Cat#406531; RRID: AB_2650758
APC anti-mouse CD154 Antibody, Clone MR1	Biolegend	Cat#106510; RRID: AB_2561561
PE/Cyanine7 anti-mouse CD62L Antibody, Clone MEL-14	Biolegend	Cat#104418; RRID: AB_313103
Alexa FluorⓇ 488 anti-mouse IL-4 Antibody, Clone 11B11	Biolegend	Cat#504109; RRID: AB_493320
APC anti-mouse/human IL-5 Antibody, Clone TRFK5	Biolegend	Cat#504306; RRID: AB_315330
IL-13 Monoclonal Antibody (eBio13A), PE-Cyanine7, eBioscience	Invitrogen	Cat#25-7133-82; RRID: AB_2573530
Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP	Thermo Fisher Scientific	Cat# G-21040, RRID:AB_2536527
SARS-CoV-2 S1_NTD-specific mAb S652-118	Vaccine Research Center, NIH	N/A
Goat Anti-Mouse IgG1, Human ads-HRP antibody	SouthernBiotech	Cat# 1070-05, RRID:AB_2650509
Goat Anti-Mouse IgG2a, Human ads-HRP antibody	SouthernBiotech	1080-05, RRID:AB_2734756
SARS-CoV-2 (COVID-19) nucleocapsid antibody	GeneTex	Cat# GTX135357, RRID:AB_2868464
EMBP (S-16) antibody	Santa Cruz Biotechnology	Cat# sc-33938, RRID:AB_2268679
Bacterial and virus strains
MA10 SARS-CoV-2	Ralph Baric Lab	N/A
Biological samples
DI SARS-CoV-1 + alum	BEI resources	Cat# NR-3882 and NR-3883
FI SARS-CoV-2	DMID, NIAID	N/A
Research-grade mRNA-1273	Moderna, Inc.	N/A
Chemicals, peptides, and recombinant proteins
Biotinylated SARS-CoV-2 S-2P conjugated to Streptavidin-APC	Vaccine Research Center, NIH	N/A
Streptavidin Allophycocyanin (APC) (Invitrogen)	Thermo Fisher Scientific	Cat#S32362
Biotinylated SARS-CoV-2 S-2P conjugated to Streptavidin-BB515	Vaccine Research Center, NIH	N/A
Streptavidin BB515	BD Biosciences	Cat# 564453, RRID:AB_2869580
Biotinylated SARS-CoV-2 RBD-SD1 conjugated to Streptavidin BV421	Vaccine Research Center, NIH	N/A
Streptavidin BV421	BD Biosciences	Cat# 563259, RRID:AB_2869475
BD Horizon Brilliant Stain Buffer	BD Biosciences	Cat#566349
eBioscience Protein Transport Inhibitor Cocktail (500X)	Invitrogen	Cat#00-4980-93
PepMix SARS-CoV-2 (Spike glycoprotein)	JPT	PM-WCPV-S
PepMix SARS-CoV-2 (NCAP)	JPT	PM-WCPV-NCAP
His-tagged SARS-CoV-2 S-2P (expressed from VRC7471_2019 nCoV S-2P-dFurin-F3CH2S_JSM)	Vaccine Research Center, NIH	N/A
KPL SureBlue TMB 1-component microwell peroxidase substrate	SureBlue	Cat#5120-0077
1N sulfuric acid	Thermo Fisher Scientific	Cat#SA212-1
Luciferase Cell Culture Lysis 5X Reagent	Promega	Cat#E1531
FuGENE® 6 Transfection Reagent	Promega	Cat#E2692
Bond Polymer Refine Detection	Leica Biosystems	Cat#DS9800
Bond Dewax Solution	Leica Biosystems	Cat#AR9222
Bond TM Epitope Retrieval 1	Leica Biosystems	Cat#AR9961
CAT Hematoxylin	Biocare Medical, Inc.	Cat#CATHE-MM
NovaUltra Eosin Solution	IHC World, LLC	Cat#IW-3100B
Critical commercial assays
Luciferase Assay System	Promega	Cat#E1501
Expifectamine 293 Transfection Kit	Thermo Fisher Scientific	Cat#A14525
BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit	BD Biosciences	Cat#554714
LIVE/DEAD Fixable Blue Dead Cell Stain Kit, for UV excitation	Invitrogen	Cat#L34962
Experimental models: Cell lines
HEK293T/17 cell line	ATCC	Cat# CRL-11268, RRID:CVCL_1926
293T cell line stably overexpressing human ACE2 cell surface receptor	Michael Farzan and Huihui Mu, Scripps Research	N/A
HEK293T/17 SF cell line	ATCC	ACS-4500, RRID:CVCL_4V93
Experimental models: Organisms/strains
BALB/cJ mouse	Jackson Laboratory	000651; RRID: IMSR_JAX:000651
Recombinant DNA
VRC5601: pHR’ CMV Luc	Naldini et al. (1996)	N/A
VRC5602: pCMV ΔR8.2	Naldini et al. (1996)	N/A
VRC9260: TMPRSS2	Vaccine Research Center, NIH	N/A
VRC7480: Spike WH-Human_epi_402119 S_VRC8400	Vaccine Research Center, NIH	N/A
Software and algorithms
FlowJo 10 software	FlowJo	N/A
Pestle software, version 2.0	Joshua Nozzi and Mario Roederer	N/A
Spice software, version 6.0	Joshua Nozzi and Mario Roederer	N/A
GraphPad Prism, version 8	GraphPad Software	N/A
Bond RX v4	Leica Biosystems	N/A
cellSens Dimension v1.9	Olympus	N/A