Fig. 3.

Mutations on the SR-rich region of N protein affect the binding potential to 14-3-3 of SARS-CoV-2 variants. Effect of S197 and surrounding amino acids mutations in N protein on the binding potential to host 14-3-3γ. The crystal Structure (PDBID: 2B05) of 14-3-3γ in complex with a phosphoserine peptide was single mutated to each amino acid of SARS-CoV-2 N protein in the corresponding position. The binding energy was calculated for each change, and determined if it was stabilizing or destabilizing by comparison with the original peptide in the crystal structure.