FIGURE 2.

Hypertrophic preconditioning suppressed I/R‐induced autophagy. A, Myocardial SIRT3 protein expression and activity. B, Myocardial acetylated SOD2 levels and activity. COX4 was used as a loading control for mitochondrial proteins. C, Quantitative analysis of DHE and MitoSOX fluorescence intensity; scale bar = 50 μm. D, The expression of LC3B in I/R‐induced myocardial tissue, as detected by immunoblotting. E, Beclin 1. F, p62. GAPDH was used as a loading control. G, Representative autophagosome images indicated by the yellow arrows, and the number of autophagosomes was quantified by analysing 15 fields in each sample; scale bar = 500 nm. n = 5‐6 per group, *P < .05