FIGURE 5.

SIRT3 deacetylated SOD2 and modulated the mROS‐autophagy pathway. A, Myocardial SOD2 protein expression and activity. B, SIRT3 protein expression and activity. C, Quantitative analysis of DHE and MitoSOX fluorescence intensity; scale bar = 50 μm. D, Quantitative analysis of LC3B expression. E, Myocardial infarct size, as detected by Evans blue and TTC staining at 24 h after I/R; Scale bar = 1 mm. F, Cardiac function (LVEF and LVFS), as measured by Doppler echocardiography at 24 h after I/R. n = 6‐8 per group, *P < .05