FIGURE 7.

Three‐dimensional culture of AM‐1 cells and effects of Wnt activators on their growth. A‐F, AM‐1 cells embedded in Matrigel (A‐D) or collagen gel (1 mg/ml for D, 2 mg/ml for F, and E as indicated) were grown in keratinocyte media with (KRCMC, 0.6 mmol/L for a‐d, 1.2 mmol/L for F, and E as indicated) or without Ca²⁺ supplement (KRCM) for 14 days. VPA was added to the media at the indicated doses (F). Scale bar = 100 μm. VPA, valproic acid