FIGURE 6.

C tsk deficiency or inhibition promotes JBMMSC proliferation and osteogenic differentiation by up‐regulating glycolysis. JBMMSC from WT mice were stimulated with 1 μmol/L DMSO, 1 μmol/L ODN, 10 μmol/L 3PO or 1 μmol/L ODN + 10 μmol/L 3PO. A, Blocking glycolysis by 3PO inhibited the effect of ODN on JBMMSC proliferation (* represent WT compared to ODN group; # represent ODN compared to 3PO+ODN group). B, Expressions of osteogenic‐related proteins of ALP and Runx2 were detected by Western blot after osteogenic induction and chemical reagent treatment for 7 d. C, Quantitative analysis of Western blot images. D, Representative images of alizarin red staining of JBMMSC after osteogenic induction and chemical reagent treatment for 15 d and quantitative analyses were shown (E). The statistical analysis was shown: *P < .05; **P < .01; ***P < .001; #P < .05; ###P < .001