FIGURE 2.

Rb1^Δ/Δp53^Δ/Δ organoid derived tumors (rb1^Δ/Δp53^Δ/Δ ODTs) recapitulate the histological features and lineage signatures of rb1^Δ/Δp53^Δ/Δ GEMM primary PCas. A, Schematic illustration of experimental design to generate ODT. B, Representative bright‐field image of rb1^Δ/Δp53^Δ/Δ ODT. C, H&E staining of the rb1^Δ/Δp53^Δ/Δ ODT section. Scale bar = 50 μm. D, Immunohistochemistry (IHC) staining reveals the expression pattern of lineage markers in rb1^Δ/Δp53^Δ/Δ GEMM primary tumor (the middle panel) and rb1^Δ/Δp53^Δ/Δ ODT sections (lower panel). WT prostate sections are used as control (upper panel). Scale bar = 50 μm. E, Quantifications of the penetrance of PCa metastasis in the rb1^Δ/Δp53^Δ/Δ GEMM organoid implanted mice (n = 20). F, Survival plot of rb1^Δ/Δp53^Δ/Δ GEMM mice (n = 9) and rb1^Δ/Δp53^Δ/Δ ODT bearing mice (n = 18). P < .0001, log rank