FIGURE 6.

A paucity of CD8⁺ T cells and abundant PD1⁺ CD4⁺ T cells further contribute to the immunesuppressive liver metastatic niche in rb1^Δ/Δp53^Δ/Δ ODT. A‐E, Representative flow cytometric analysis and quantification of the ratio of B cells (A), CD3⁺ T cells (B), CD8⁺ T cells (C) and CD4⁺ T cells (D) in CD45⁺ mCherry⁺ cells, and PD1⁺ CD4⁺ T cells (E) in CD45⁺ mCherry⁺ CD4⁺ T cells in rb1^Δ/Δp53^Δ/Δ ODT liver metastasis niche at the early, middle, and late stages. Unpaired two‐tailed Student's t‐test is used for statistical analysis. The data are presented as the means ± SEMs. *P < .05, **P < .01, n = 5