FIGURE 5.

PPARγ is essential for the inhibitory role of KLF14 overexpression in HSCs. A, For PPARγ inhibition, the LX‐2‐control and LX‐2‐KLF14 cells were treated with GW9662 (1 μmol/L) for 24 h or stably transfected with LV‐shPPARγ. The expression of KLF14 and PPARγ was assessed by Western blotting analysis. B, Lipid droplets accumulation was measured by Oil Red O staining (Scale bars: 20 μm). C, Expression of α‐SMA and COL1A1 was measured by RT‐qPCR and Western blotting analyses. D, Cell proliferation was assessed by CCK‐8 assay. (E,F) Cell cycle and cell apoptosis were detected by flow cytometry. G, Cell migration ability was measured by Transwell migration assay (Scale bars: 100 μm). **P < .01, ***P < .001, ns, P > .05 versus the control group. ^## P < .01, ^### P < .001 versus the KLF14 group. n = 3