TABLE 5.
Summary of immune-relevant genes characterized from sick (vs. healthy) barramundi based on KEGG functional analysis.
| Gene family | Gene name |
Liver |
Muscle |
Gene description | Gene ID | ||||
| Log2 fold change | P (adj) | Up/downregulated | Log2 fold change | P (adj) | Up/downregulated | ||||
| Toll-like receptor | tlr5 | 2.83 | 0.012439 | Up | 7.50 | 0.00171 | Up | Toll-like receptor 5 | LOC108888912 |
| tlr13 | 5.80 | 5.26E-24 | Up | 9.14 | 9.88E-10 | Up | Toll-like receptor 13 | LOC108885100 | |
| Lectin | cl-11l | –1.33 | 0.004857 | Down | –a | –a | –a | Collectin-11-like | LOC108889727 |
| colec12 | 2.60 | 0.00014 | Up | –a | –a | –a | Collectin subfamily member 12 | LOC108883269 | |
| selp | 2.76 | 2.82E-06 | Up | –a | –a | –a | Selectin P | LOC108877420 | |
| Complement | C1ql4 | –6.21 | 9.61E-09 | Down | –a | –a | –a | Complement C1q-like protein 4 | LOC108891007 |
| C3 | –2.51 | 0.001909 | Down | –a | –a | –a | Complement C3-like | LOC108885851 | |
| C5 | 1.59 | 0.001243 | Up | –a | –a | –a | Complement C5 | C5 | |
| C6 | 3.24 | 1.41E-13 | Up | –a | –a | –a | Complement C6 | C6 | |
| CTRP | ctrp1 | –7.91 | 4.23E-17 | Down | –10.12 | 0.038363 | Down | Complement C1q tumor necrosis factor-related protein 1-like | LOC108890739 |
| Chemokine | ccl4 | –3.15 | 0.000577 | Down | –a | –a | –a | C-C motif chemokine 4 homolog | LOC108890197 |
| ccl25b | 0.92 | 0.04234 | Up | –a | –a | –a | Chemokine (C–C motif) ligand 25b | LOC108881384 | |
| c-x-cl12a | 0.99 | 0.031141 | Up | –a | –a | –a | Chemokine (C–X–C motif) ligand 12a (stromal cell-derived factor 1) | LOC108888279 | |
| ccr7 | –a | –a | –a | –2.93 | 0.046610 | Down | Chemokine (C–C motif) receptor 7 | ccr7 | |
| cxcr3l | 1.23 | 0.007315 | Up | –a | –a | –a | C–X–C chemokine receptor type 3-like | LOC108880701 | |
| ackr3b | 1.09 | 0.015943 | Up | –a | –a | –a | Atypical chemokine receptor 3b | ackr3 | |
| ccr6b | –1.77 | 0.000066 | Down | –a | –a | –a | Chemokine (C–C motif) receptor 6b | LOC108896100 | |
| Interleukin | il-1β | 3.91 | 5.09E-06 | Up | –a | –a | –a | Interleukin-1 beta-like | LOC108878374 |
| irak4 | 1.24 | 0.007064 | Up | –a | –a | –a | Interleukin-1 receptor-associated kinase 4 | irak4 | |
| il11a | 3.08 | 0.000064 | Up | –a | –a | –a | Interleukin 11a | LOC108887322 | |
| il13rα1 | 1.75 | 0.000044 | Up | –a | –a | –a | Interleukin 13 receptor, alpha 1 | LOC108882024 | |
| il1r1l | 1.02 | 0.031425 | Up | –a | –a | –a | Interleukin-1 receptor type 1-like | LOC108897039 | |
| il17ra | –1.33 | 0.031206 | Down | –a | –a | –a | Interleukin-17 receptor A-like | LOC108888530 | |
| Il7R-αl | –1.37 | 0.002399 | Down | –a | –a | –a | Interleukin-7 receptor subunit alpha-like | LOC108892301 | |
| Tumor necrosis factor (TNF) | tnfrsf11a | 1.89 | 0.013310 | Up | –a | –a | –a | Tumor necrosis factor receptor superfamily, member 11a, NFKB activator | LOC108900798 |
| tnfaip3 | 1.78 | 0.000031 | Up | –a | –a | –a | Tumor necrosis factor, alpha-induced protein 3 | tnfaip3 | |
| tnfsf10l | –2.05 | 0.003045 | Down | –a | –a | –a | Tumor necrosis factor ligand superfamily member 10-like | LOC108885067 | |
| tnfsf12 | –2.54 | 0.000000 | Down | –3.86 | 0.002753 | Down | TNF superfamily member 12 | LOC108902465 | |
| Collagen | col4a5 | –3.78 | 1.87E-08 | Down | –a | –a | Down | Collagen alpha-5 (IV) chain-like | LOC108883501 |
| col6a6 | –1.78 | 0.046855 | Down | –5.04 | 0.000399 | Down | Collagen alpha-6 (VI) chain-like | LOC108881847 | |
| col6a6 | –3.93 | 0.000086 | Down | –4.43 | 0.001080 | Down | Collagen alpha-6 (VI) chain-like | LOC108881842 | |
| col6a3 | –1.52 | 0.027935 | Down | –a | –a | Down | Collagen type VI alpha 3 chain | col6a3 | |
| col1a1b | –3.32 | 0.000073 | Down | –3.63 | 0.004069 | Down | Collagen, type I, alpha 1b | LOC108876589 | |
| col1a2 | –3.80 | 0.000046 | Down | –3.69 | 0.003305 | Down | Collagen, type I, alpha 2 | col1a2 | |
| col6a1 | –1.75 | 0.000495 | Down | –3.79 | 0.001714 | Down | Collagen, type VI, alpha 1 | col6a1 | |
| col6a2 | –2.18 | 1.56E-06 | Down | –2.81 | 0.046610 | Down | Collagen, type VI, alpha 2 | LOC108883902 | |
| col2a1b | –a | –a | Down | –2.96 | 0.036736 | Down | Collagen type II alpha 1b | LOC108884852 | |
| col1a1a | –3.94 | 0.000021 | Down | –4.67 | 0.007110 | Down | Collagen, type I, alpha 1a | LOC108898561 | |
| col6a6 | –a | –a | Down | –3.82 | 0.005404 | Down | Collagen, type VI, alpha 6 | col6a6 | |
| Glutathione S-transferase (gst) | gstm3l | –1.22 | 0.004676 | Down | –a | –a | –a | Glutathione S-transferase Mu 3-like | LOC108876447 |
| gsta.1 | –1.96 | 0.038464 | Down | –a | –a | –a | Glutathione S-transferase, alpha tandem duplicate 1 | LOC108891126 | |
| mgst3a | –1.28 | 0.002452 | Down | –a | –a | –a | Microsomal glutathione S-transferase 3a | mgst3a | |
| CD molecules | myd88 | 1.15 | 0.010149 | Up | –a | –a | –a | MYD88 innate immune signal transduction adaptor | myd88 |
| cd276 | 1.27 | 0.008465 | Up | –a | –a | –a | CD276 molecule | cd276 | |
| cd74 | –1.64 | 0.000159 | Down | –a | –a | –a | CD74 molecule | cd74 | |
| cd276 | –1.05 | 0.038810 | Down | –a | –a | –a | CD276 antigen-like | LOC108886627 | |
| cd34 | –a | –a | –a | –3.89 | 0.018911 | Down | CD34 molecule | cd34 | |
| cd166 | –a | –a | –a | –2.95 | 0.021215 | Down | CD166 antigen homolog A-like | LOC108899782 | |
| cd248 | –a | –a | –a | –6.50 | 0.00630 | Down | CD248 molecule | cd248 | |
| B cell | cd22 | –1.15 | 0.017344 | Down | –a | –a | –a | B-Cell receptor CD22-like | LOC108880790 |
| T cell | tcirg1 | 1.25 | 0.006142 | Up | –a | –a | –a | T-Cell immune regulator 1 | tcirg1 |
| nfatc1 | –1.97 | 0.010264 | Down | –a | –a | –a | Nuclear factor of activated T cells 1 | LOC108899182 | |
| Major histocompatibility complex (MHC) | h2-aa | –2.37 | 0.027046 | Down | –a | –a | –a | H-2 class II histocompatibility antigen, A-U alpha chain-like | LOC108892329 |
| h2-eb1 | –1.60 | 0.016974 | Down | –a | –a | –a | H-2 class II histocompatibility antigen, E-D beta chain-like | LOC108890468 | |
| h2-eb1 | –1.97 | 0.008825 | Down | –a | –a | –a | H-2 class II histocompatibility antigen, E-S beta chain-like | LOC108892328 | |
| h2-eb1 | –2.62 | 6.93E-06 | Down | –a | –a | –a | H-2 class II histocompatibility antigen, E-S beta chain-like | LOC108882204 | |
| h2-eb1 | –3.02 | 0.000078 | Down | –a | –a | –a | H-2 class II histocompatibility antigen, E-S beta chain-like | LOC108882336 | |
| hla-dap1 | –1.67 | 0.000442 | Down | –a | –a | –a | HLA class II histocompatibility antigen, DP alpha 1 chain-like | LOC108882210 | |
aNo differential expression of the gene in liver or muscle.