TABLE 1.
Mediators of innate and adaptive immunity released by MΦ in response to CoV‐2
| CF | Non‐CF | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Ctrl | S1 | Ctrl | S1 | ||||||||
| Mean | SE | Mean | SE | P vs Ctrl | Mean | SE | Mean | SE | P vs Ctrl | P CF +S1 vs Non‐CF +S1 | |
| IL‐8 | 2.1 | 0.7 | 1516.0 | 919.4 | * | 19.74 | 5.35 | 1272.28 | 323.68 | * | ns |
| IL‐1RA | 3092.8 | 353.4 | 2234.3 | 52.7 | * | 283.78 | 45.69 | 300.93 | 53.10 | ns | ** |
| GM‐CSF | 0.0 | 0.0 | 6.2 | 3.2 | * | 0.0 | 0.0 | 7.5 | 2.5 | * | ns |
| MCP‐1 | 10.2 | 4.5 | 107.6 | 24.5 | * | 11.00 | 1.36 | 2757.40 | 550.44 | * | *** |
| MIP‐1 alpha | 14.6 | 10.4 | 532.0 | 58.8 | * | 9.94 | 3.79 | 501.58 | 121.59 | * | ns |
| MIP‐1 beta | 4.4 | 4.1 | 292.4 | 21.6 | *** | 7.84 | 2.25 | 1843.81 | 635.47 | * | * |
| RANTES | 10.1 | 0.5 | 16.7 | 5.0 | * | 5.54 | 0.93 | 26.86 | 15.54 | ns | ns |
| TNF alpha | nd | ‒ | nd | ‒ | ‒ | 0.0 | 0.0 | 1011.68 | 130.42 | * | ns |
| VEGF | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| EGF | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| EOTAXIN | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‐ | ‒ | ‒ |
| FGF‐2 | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‐ | ‒ |
| G‐CSF | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| HGF | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IFN alpha | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IFN gamma | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IL‐1 beta | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IL‐10 | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IL‐12 | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IL‐13 | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IL‐15 | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IL‐17A | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IL‐2 | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IL‐2R | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IL‐4 | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IL‐5 | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IL‐6 | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IL‒7 | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| IP‒10 | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
| MIG | nd | ‒ | nd | ‒ | ‒ | nd | ‒ | nd | ‒ | ‒ | ‒ |
Concentrations of proteins involved in innate and adaptive immune response released by monocyte‐derived MΦ isolated from volunteers with and without CF upon treatment (3 h, 37 °C) with recombinant CoV‐2 spike proteins subunit 1 (S1, 10 µg/mL). Protein concentrations were measured using a multiplex Luminex array kit. Results are reported as pg/mL and are of experiments will cells from four different donors. nd, not detected (below limit). ns, not significant; *P < .05; **P < .01; ***P < .001 (One‐Way ANOVA).