TABLE 2.
Expansion results
| Parameter | Donor 1 | Donor 2 | Donor 3 | |||||
|---|---|---|---|---|---|---|---|---|
| Day 7 | Fold expansion | 0.76x | 0.63x | 0.36x | ||||
| Day 14 | Fold expansion | 1.6x | 1.8x | 0.96x | ||||
|
Specificity (% TNFα/IFNγ) |
CD4+ | Pep. M + N+S | 1.4 | 16.7 | 0 | |||
| CD8+ | Pep. M + N+S | 0 | 27.0 | 0 | ||||
| Day 21 | Fold expansion | 1.6x | 1.2x | |||||
|
Product identity (% of total) |
CD3+ | 89.3 | 62.7 | |||||
| CD4+ (% of CD3) | 60.1 | 58.6 | ||||||
| CD8+ (% of CD3) | 35.0 | 37.4 | ||||||
| CD56+ | 2.23 | 33.9 | ||||||
| CD19+ | 2.59 | 0.33 | ||||||
| CD14+ | 5.42 | 0.22 | ||||||
|
Cytotoxic Potential (% CD107a) |
CD4+ | Pep. M | 3.3 | 5.0 | ||||
| Pep. N | 0 | 0 | ||||||
| Pep. S | 12.8 | 20.4 | ||||||
| CD8+ | Pep. M | 0 | 12.7 | |||||
| Pep. N | 0 | 0 | ||||||
| Pep. S | 15.1 | 30.0 | ||||||
| Sterility | OK | OK | ||||||
Summary of the cultures cell growth number, composition, and specificity. For donor number 3, culture was ended on day 14 because no SARS‐CoV‐2 specificity could be identified.