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. 2021 Apr 28;24(3):315–329. doi: 10.4048/jbc.2021.24.e24

Figure 4. The HAGLR/miR-93-5p/SRSF1 axis contributed to the malignance of TNBC cells.

Figure 4

(A, B) The knockdown efficacy of the miR-93-5p inhibitor and the overexpression efficacy of pcDNA3.1-SRSF1 were analyzed by qRT-PCR. (C, D) Immunofluorescence and colony formation assays were implemented to investigate the proliferation of the indicated MDA-MB-436 and HCC-1937 cells. (E-G) JC-1 assay, TUNEL assay, and western blot were implemented to detect cell apoptosis under different circumstances. (H) A Transwell assay was carried out to determine the migration and invasion of the indicated MDA-MB-436 and HCC-1937 cells.

HAGLR = HOXD antisense growth-associated lncRNA; miR = microRNA; SRSF1 = serine- and arginine-rich splicing factor 1; TNBC = triple-negative breast cancer; qRT-PCR = quantitative real-time polymerase chain reaction; NC = negative control; GAPDH = glyceraldehyde 3-phosphate dehydrogenase.

**p < 0.01.