Figure 5. RNAPIIS5P CUTAC shows high sensitivity and specificity.

Mapped fragments from the indicated K562 datasets were sampled, and peaks were called using MACS2. A) Number of peaks (left) and B) fraction of reads in peaks for CUT&Tag (triangles) and CUTAC (squares) profiles for H3K4me2, RNAPIIS5 (initiation form), RNAPIIS2P (elongation form), RNAPIIS2P/S5P, and Omni-ATAC (green). CUTAC for RNAPIIS5P shows the best sensitivity (most peaks at low sampling) and the best signal-to-noise (highest FRiP at all sampling levels). Tagmentation was for 10 min at 37°C in CUTAC-tag buffer.