TABLE 4.
Key enzymes involved in biosynthesis of volatile organic compounds acetoin and 2,3-butanediol.
| $ | $ | $ | $ | $ | $ | $ | ¤ | ¤ | ¤ | ¤ | ¤ | ||
| Pphy | Pxen | Puna | Pban | Psac | Sui | Bamb | Bvie | Bglu | Bmal | Bpse | Bcen | Bcep | |
| Pyruvate decarboxylase | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Acetolactate synthase | 3 | 7 | 3 | 4 | 4 | 4 | 3 | 3 | 3 | 2 | 3 | 3 | 4 |
| Acetoin dehydrogenase | 1 | 1 | 2 | 1 | 1 | 3 | 1 | 1 | – | – | – | 1 | – |
| Acetoin reductase | – | – | 1 | – | – | 1 | – | – | – | – | – | – | – |
Enzyme sequences from genome annotation (swissport) of Paraburkholderia sacchari Suichang626 were used as references. BLASTp was conducted against all annotations. Selection criteria are e-value < 1e-50, and similarity >40%. Protein sequence of acetolactate decarboxylase (Paraburkholderia sp. DHOC27 WP117337522.1) was searched and no hit was found from all tested species. Pphy, P. phytofirmans; Pxen, P. xenovorans; Puna, P. unamae; Pban, P. bannensis; Psac, P. sacchari; Sui, P. sacchari Suichang626; Bamb, B. ambifaria; Bvie, B. vietnamiensis; Bglu, B. glumae; Bmal, B. mallei; Bpse, B. pseudomallei; Bcen, B. cenocepacia; Bcep, B. cepacia. §, plant beneficial bacteria; ¤, plant/animal pathogen.