FIGURE 3.

Evaluation of the toxicity of quercetin and isorhamnetin on ACE2^h cells by the change of cell viability and intracellular Ca²⁺ flux. (a and b) Effect of quercetin (a) and isorhamnetin (b) on the viability of ACE2^h cells. ACE2^h cells were treated with different concentrations of quercetin and isorhamnetin for 24 h, cells viability was tested by CCK8 assay. (c and d) Effect of quercetin (c) and isorhamnetin (d) on intracellular Ca²⁺ flux change in ACE2^h cells. ACE2^h cells were pretreated with 100 μM concentrations of quercetin and isorhamnetin, intracellular Ca²⁺ flux was tested by Ca²⁺ mobilization assay. The experiments were repeated three times. Data are presented as mean ± SEM