FIGURE 3.

Modified siRNA have increased resistance to nuclease degradation. A, The stability of unmodified siR‐7 (circles) and modified siR‐7‐EM (squares) in 50% mouse serum are compared over a period of 264 h at 37°C (N = 4). siRNA quantities at various time points were calculated by dividing the total counts of full‐length siRNA by the input starting material. B, For this purpose, aliquots of each sample (10 μg siRNA per lane) were analyzed by 1.5% agarose gel electrophoresis. Differences between multiple groups were analyzed using repeated measures one‐way ANOVA with Dunnetts's post hoc test. Bars show medians of four independent experiments+SEM. **** p < 0.0001