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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Methods Mol Biol. 2021;2262:271–280. doi: 10.1007/978-1-0716-1190-6_16

Fig. 1.

Fig. 1

A flow-chart showing the overall method to perfom RAS BioID. HEK-HT cells are transfected with myc-BirA*-HRASG12V, myc-BirA*-NRASG12V and myc-BirA*-KRASG12V plasmid constructs in three biological replicates. Exogenous biotin is added to the cells and incubated for 24hrs. Cells are then lysed and subjected to streptavidin affinity capture and eluates are processed for mass-spectrometry analysis for detection of protein interactomes of RAS oncoproteins. All samples are processed in three independent biological replicates until the mass-spectrometry step. The proteomic dataset is analyzed for components that are shared or uniquely identified in all the three RAS isoform interactomes.