Figure 1.

IDO1 is among the genes that best defines peripheral blood cDC1s under homeostatic conditions and its expression is increased upon stimulation with TLR agonists. Gene expression was analyzed from RNA‐seq datasets. Z scores were calculated using normalized and log transformed counts per million values. Means are depicted. (A) Expression of selected genes was compared between immature cDC1s, immature cDC2s, and immature (unstimulated or IL‐3‐treated) pDCs, of 4 (cDC1) or 3 (cDC2 and pDC) different donors. (B) IDO1 gene expression among cDC1s of three or four different donors cultured overnight in absence or presence of a clinical grade TLR3 agonist (Hiltonol) and/or TLR7/8 agonist (protamine‐RNA).