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a) Recombinant expression of fusion protein F and conversion to disulfide‐protected fragment C; b) native chemical ligation of segments A and B3–B9 to IL‐6 (1–48) hydrazides D3–D9 and conversion to thioesters E3–E9; c) native chemical ligation of thioesters E3–E9 with segment C followed by a two‐step refolding and oxidation of the full‐length glycopeptides H3–H9 to the IL‐6 glycoforms IL‐63–IL‐69.
