TABLE 1.

Quantitative data describing duplex sequencing mutation analysis of EMS‐treated human ALI airway tissue models

Treatment	Replicate	Total duplex bases sequenced (×106)	Mutant nucleotides detected	Mutant frequency (×10−7)
Vehicle control	1	481	76	1.58
	2	513	75	1.46
	3	636	156	2.45
	4	727	131	1.80
Mean		589	110	1.86
6.25 μg/mL	1	796	189	2.37
	2	323	64	1.98
	3	709	174	2.45
Mean		609	142	2.27
25 μg/mL	1	699	220	3.15
	2	726	244	3.36
	3	725	205	2.83
Mean		717	223	3.11
50 μg/mL	1	444	161	3.62
	2	456	172	3.77
	3	685	300	4.38
	4	642	280	4.36
Mean		557	228	4.03
100 μg/mL	1	423	423	10.00
	2	398	325	8.17
	3	746	875	11.73
	4	407	458	11.24
Mean		494	520	10.28