Figure 2.

MBD3 expression peaks at the climax of metamorphosis in the intestine and tail during natural metamorphosis. Intestinal (A) and tail (B) RNAs were extracted from metamorphosing tadpoles and subjected to cDNA synthesis. qRT-PCR was conducted to examine the expression of MBD3 and control gene EF1α, respectively. MBD3 expression was normalized against that of EF1α in the same cDNA samples and presented as mean +/− standard error of the mean (S.E.M). One-way ANOVA followed by Boferroni’s test was done to compare the samples (* p<0.05).