FIGURE 1.

Netrin‐1 is significantly upregulated in endotoxin‐treated myeloid cells. A, Monocytes were isolated from blood of healthy donors and differentiated into macrophages (MΦs) then treated with LPS (1 µg/mL for 8 hours). RNA was isolated and used to perform a neuron guidance‐specific RT‐qPCR array. B, The top upregulated genes from the array demonstrated the netrin‐1 gene (NTN1) as the top‐statistically significant induced gene (n = 4, Bonferroni adjusted unpaired or Welch’s t test). C, PCR confirmation of NTN1 upregulation in LPS stimulated human blood‐derived MΦs (n = 3 per group, one‐way ANOVA with Dunnett’s post hoc tests). D and E, Representative image and quantification for Western blot for netrin‐1 protein expression in LPS‐treated MΦs (n = 3 per group, one‐way ANOVA with Dunnett’s post hoc tests). F, Peripheral polymorphonuclear neutrophils (PMNs) were isolated from healthy donors and treated with 1 µg/mL of LPS for 8 hours. G, NTN1 RT‐qPCR using RNA isolation from LPS‐treated PMNs (n = 3 per group, unpaired t test). H and I, Representative image and quantification for Western blot for netrin‐1 protein expression in LPS‐treated PMNs (n = 3, unpaired t test). All data are represented as mean ± SD; *P‐value < .05