FIGURE 4.

The combination of BFOV and metformin improved hepatic inflammatory state and energy metabolism via downregulating inflammatory signaling pathways and activating phosphorylation of AMPK and AKT in the liver of HFC57 mice. (A) Hepatic NO content. (B) Hepatic iNOS activity. (C‐E) Representative bands and quantitative analysis of main factors involving in the inflammatory pathway in the liver by Western blot, including (C) NFκB‐p65 (65KD), (D) iNOS (130KD) and (E) MMP‐2 (72KD). (F, G) Representative bands and quantitative analysis of ratio of phosphorylation AMPKα (62KD) and phosphorylation AKT (60KD) expression in liver by Western blot, each phosphorylation was normalized by the total amount of (F) AMPK (62KD), (G) AKT (60KD). β‐Actin served as a loading control. Data represented the mean of at least three independent experiments ± SEM. One‐way ANOVA: * P <.05, ** P <.01, *** P <.001 vs. Con. ^# P <.05, ^## P <.01, ^### P <.001 vs. BFOV + Met