Figure 2.

Docetaxel response in prostate cancer cell line upon AdipoCM treatment. DU145 and PC3 (2 × 10⁴ cells) cells were plated in 96 well plates and serum starved for 16 h cells. The cells were incubated with 0.25% BSA or PPAT Adipocyte‐CM from Adeno, G7(3 + 4) and G8(4 + 4) adipocytes for 24 h. Then, the cells were treated with docetaxel 8 nM for 48 h alone or in combination with AdipoCM. Cell viability was assessed by the MTT assay. The results were reported as percentage of viable cells compared to control, considered as maximum viability (100%). Data represent the mean ± SD of triplicate samples of three independent experiments. *p value < .05. BSA, bovine serum albumin; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; PPAT, periprostatic adipose tissue; SD, standard deviation