Figure 6.

AG1024 effect on TUBB2B expression in prostate cancer cell. (A) DU145 and (B) PC3 were serum starved for 16 h cells and then incubated with 0,25% BSA or PPAT Adipocyte‐CM from Adeno and G8(4 + 4) adipocytes and in combination with AG1024 8 µM. (C) DU145 and (D) PC3 cells were serum starved for 16 h, pretreated with AG1024 8 µM and then stimulated with IGF‐1 recombinant protein for 48 h. The lysates were analyzed by immunoblotting with pAKT and TUBB2B antibodies and autoradiography. GAPDH antibody was used for normalization. The autoradiograph shown is representative of three different experiments. BSA, bovine serum albumin; PPAT, periprostatic adipose tissue; TUBB2B, β‐tubulin isoform 2B