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. 2021 Mar 10;106(2):555–565. doi: 10.1111/tpj.15164

Figure 1.

Figure 1

PVX vector to express sgRNAs for CRISPR‐Cas9‐based gene editing in Nicotiana benthamiana. Left panel, schematic representation of recombinant clones PVX::tR‐sgRNA‐tR, PVX::tR‐sgRNA, PVX::sgRNA‐tR and PVX::sgRNA, which express different versions of a sgRNA flanked or not with tRNAs to promote RNA processing by endogenous RNases. Right panel, structure of the subgenomic primary transcripts of each PVX recombinant clone. RdRp, RNA‐dependent RNA polymerase; TGB, triple gene block; and CP, coat protein, are represented by open boxes. Heterologous Bamboo mosaic virus CP promoter (CPBaMV) is represented by a black arrow. 5′ and 3′ untranslated regions (UTRs) are represented by lines. tRNA sequences are represented by round, light blue boxes. sgRNA consists of a gene‐specific 20‐nt protospacer (orange diamond) and a conserved 76‐nt scaffold (gray box). CPBaMV, tRNAs, protospacer and scaffold, not at scale.