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. 2021 Mar 10;106(2):555–565. doi: 10.1111/tpj.15164

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© 2021 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Single versus multiplex gene editing using the PVX‐based system in Nicotiana benthamiana.

(a) Schematic representation of recombinant clones PVX::sgFT (single sgRNA strategy) and PVX::sgPDS3:sgFT:sgXT2B (multiplex sgRNA strategy). Protospacers for NbPDS3, NbFT and NbXT2B are represented by blue, pink and green diamonds, respectively. Other details as in the legend to Figure 1.

(b) ICE analysis of the first systemically infected upper leaf of N. benthamiana plants (n = 4) inoculated with PVX::sgFT (left) and PVX::sgPDS3:sgFT:sgXT2B (right). PVX::crtB was used as a negative control. Columns and error bars represent average indels (%) and standard deviation, respectively. dpi, days post‐inoculation.