Figure 5.

Heritable gene editing using the PVX‐based system in Nicotiana benthamiana.

(a) ICE analysis of the progeny of plants 14.3 (left) and 14.1 (right), regenerated from leaf discs infected with PVX::sgXT2B and PVX::sgPDS3:sgFT:sgXT2B, respectively. Individual plants of the progeny (n = 10) are numbered i–x. Columns represent average indels (%).

(b) Genotype analysis of the same progeny (n = 10) used to determine heritable gene editing in (a). Heterozygous progeny contain one wild‐type allele and one edited allele; biallelic progeny contain both alleles edited. Percentage of each genotype is represented in stacked columns and was determined by the fraction of progeny containing that genotype divided by total progeny assessed.