Figure 7.

Knockdown of SMO suppresses AR signaling via Gli1. (A) LNCaP and C4‐2B cells were treated with an Hh antagonist (GANT61) or Hh activator (SAG) for 48 h, the expression of AR and PSA was assessed by western blot and ELISA. (B) sh‐SMO or sh‐NC LNCaP and C4‐2B cells were transfected with ectopic recombinant Gli1 cDNA (oe‐GLI1), and the expression of Gli1, AR, and PSA was assessed by western blot. AR, androgen receptor; cDNA, complementary DNA; NC, negative control; PSA, prostate‐specific antigen; SMO, smoothened