Figure 5.

Editing efficiency of the EGFP gene and EMX1 gene in two human cell lines. The insertions and deletions (indel) frequency of both EGFP transgene (702 bp; cleaved to 383 bp and 319 bp) and human EMX1 gene (593 bp; cleaved to 300 bp and 293 bp) were measured in a) Hela‐GFP cells, b) HEK293‐GFP cells. The indel frequency was calculated by dividing the intensity of the fragment bands by that of the PCR input band. The indel frequency of the EMX1 gene using the RNP corona was compared with those using lipid‐mediated delivery method (CRISPR‐Max) in HEK293‐GFP cells.