Figure 3.

Evaluation of the different STxB and TAT conjugates for the Cyto‐SNAP assay. a,b) Comparison of the ELISA signal obtained with the different STxB and TAT conjugates directly added into cell lysate. 40 pm of each STxB conjugate or 100 pm of each TAT conjugate were added to NG‐SNAP cell lysate and incubated for 1 h at 37 °C for the SNAP reaction to occur before mNeonGreen immunoprecipitation and ELISA development on beads. c,d) Comparison of cytosolic arrival signal from the different STxB conjugates (40 nm) or TAT conjugates (200 nm) after 4 h incubation at 37 °C with NG or NG‐SNAP HeLa cells.