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. 2021 Apr 6;16(12):1956–1966. doi: 10.1002/cmdc.202100075

Figure 5.

Figure 5

Effects of the platinum complexes on the cellular thiol redox systems. Cells (250 000/well) were treated with 5 and 10 μm 1 and 2 or cis‐Pt for 48 h with a refresh after 24 h. A) Total free thiols titrated with DTNB as described in the Experimental Section and expressed as a percentage with respect to the control. B) Total glutathione (GSH+GSSG) and oxidized glutathione (GSSG) levels in A2780 and A2780cis cells reported as nmol/mg protein. C) Total TrxR activity and D) total GR activity estimated spectrophotometrically and reported as nmol/min/mg protein. * p<0.05, ** p<0.01, *** p<0.001. Mean values ±SD of four experiments are reported.