Fig. 4.

Methylation levels at candidate autosomal loci between 46,XY and 49,XXXXY. Genomic DNA extracted from saliva was used for bisulfite pyrosequencing of putative regulatory regions at two genes involved in neurodevelopment and neurotransmission: GABA type A receptor alpha5 subunit (GABRA5) on 15q12 (a) and SH3 multiple ankyrin repeat domains 3 (SHANK3) on 22q13 (b). Error bars are presented as mean ± STD. *P < 0.05