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. 2021 Jul 2;14:149. doi: 10.1186/s13068-021-02002-z

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Comparison of partial promoter strength with isoamyl alcohol titer. Several promoters that cover a range of strengths (left) were selected to replace P_UAS1B4-LEUm for overexpressing ScARO10 and the titers of isomayl alcohol were measured (right). The strain with P_UAS1B4-EXPm achieved the highest isoamyl alcohol titer of 11.57 mg/L, which was approximately 30.3-fold higher than that of Po1g KU70Δ and 5.4-fold that of Po1g BAA. The error bars represent standard deviation