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. 2021 Jul 1;11:120. doi: 10.1186/s13578-021-00631-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Chemerin induced phosphorylation of JNK1/2, ERK1/2, and p38 and increased the expression of migration-related genes in hBM-MSCs. A Western blot of phosphorylated JNK1/2, p38, and ERK1/2 in hBM-MSCs after chemerin treatment. Protein expression was quantified using the Image J software. Expression was normalized to that of GAPDH (N > 3). B RT-qPCR of CD44, ITGA4, and MMP-2 after chemerin treatment. mRNA levels were observed 24 h after the chemerin treatment of BM-MSCs. C Western blot analysis of CD44, ITGA4, and MMP-2 of hBM-MSCs after chemerin treatment. Protein expression was quantified using Image J and normalized to GAPDH level. D Venn diagram of common transcription factors, CD44, ITGA4, and MMP2. Transcription of each gene was found using GP miner and 26 genes were found to encode common transcription factors. Among these transcription factors, five were involved in transmigration. GP miner (http://gpminer.mbc.nctu.edu.tw/). E RT-qPCR of transcription factors in naïve and chemerin-treated hBM-MSCs. The data shown were reproducible results from three independent experiments

Fig. 2 — Chemerin induced phosphorylation of JNK1/2, ERK1/2, and p38 and increased the expression of migration-related genes in hBM-MSCs. A Western blot of phosphorylated JNK1/2, p38, and ERK1/2 in hBM-MSCs after chemerin treatment. Protein expression was quantified using the Image J software. Expression was normalized to that of GAPDH (N > 3). B RT-qPCR of CD44, ITGA4, and MMP-2 after chemerin treatment. mRNA levels were observed 24 h after the chemerin treatment of BM-MSCs. C Western blot analysis of CD44, ITGA4, and MMP-2 of hBM-MSCs after chemerin treatment. Protein expression was quantified using Image J and normalized to GAPDH level. D Venn diagram of common transcription factors, CD44, ITGA4, and MMP2. Transcription of each gene was found using GP miner and 26 genes were found to encode common transcription factors. Among these transcription factors, five were involved in transmigration. GP miner (http://gpminer.mbc.nctu.edu.tw/). E RT-qPCR of transcription factors in naïve and chemerin-treated hBM-MSCs. The data shown were reproducible results from three independent experiments