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. 2021 Mar 18;230(6):2186–2199. doi: 10.1111/nph.17264

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© 2021 The Authors New Phytologist © 2021 New Phytologist Foundation

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Fig. 5 — Comparison of laccase‐mediated and peroxidase‐mediated incorporation of fluorescence‐tagged monolignols into differentiating compression and normal wood tracheids of Chamaecyparis obtusa seedlings. (a, b) Serial transverse sections from compression (a) and normal (b) wood tissues were labelled with DMAC‐tagged monolignols (DMAC‐PA and DMAC‐CA) in the presence of catalase for detection of laccase/O₂ (LACs), glucose and glucose oxidase for peroxidase/H₂O₂ (PRXs), and with H₂O₂ for both laccase/O₂ and peroxidase/H₂O₂ (LACs + PRXs) oxidation activities. Regions where the incorporation of fluorescence‐tagged monolignols was detected are indicated on the heat control sections. Bars, 20 μm. (c) Normalised DMAC florescence determined for where the incorporation of fluorescence‐tagged monolignols was detected in different cell wall compartments. Values are based on the mean fluorescence intensity in grey levels ± SD (n ≧ 5). Different letters indicate significant differences (ANOVA with post‐hoc Tukey–Kramer test, P < 0.05).