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. 2021 Mar 10;14(8):1781–1804. doi: 10.1002/cssc.202100159

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© 2021 The Authors. ChemSusChem published by Wiley-VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Scheme 5 — Regioselective β‐carboxylation of (p‐hydroxy)styrenes (a) Regioselective β‐carboxylation of para‐hydroxystyrenes by applying E. coli whole cells expressing co‐factor free phenolic acid decarboxylases (PADs)/ferulic acid decarboxylase (FDC) from bacterial sources. ^[52] Enzymes: Phenolic acid decarboxylase (PAD) from Bacillus amyloliquefaciens (PAD_Ba); from Mycobacterium colombiense (PAD_Mc), and ferulic acid decarboxylase (FDC) from Enterobacter sp. (FDC_Es). For clarity, conversion values (%) obtained with PAD_Ba, PAD_Mc and FDC_Es have been given in black, blue, and pink, respectively. (b) Regioselective β‐carboxylation of styrene catalyzed by prFMN‐bound ferulic acid decarboxylase from Aspergillus niger (AnFdc). ^[43]